{"id":200,"date":"2019-12-13T05:48:25","date_gmt":"2019-12-13T05:48:25","guid":{"rendered":"http:\/\/sites.gtiit.edu.cn\/chemanalyinstrucore\/?page_id=200"},"modified":"2025-12-05T06:13:45","modified_gmt":"2025-12-05T06:13:45","slug":"maldi-tof","status":"publish","type":"page","link":"https:\/\/sites.gtiit.edu.cn\/chemanalyinstrucore\/maldi-tof\/","title":{"rendered":"maldi-tof"},"content":{"rendered":"\t\t
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<\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Bruker Autoflex Max LRF Maldi-TOF<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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The Matrix-Assisted Laser Desorption\/Ionization Time-of-Flight (MALDI-TOF) is a powerful mass spectrometer with a rapid, accurate, and high-resolution technique. The MALDI process involves sample molecules crystallising with a matrix compound, which upon laser irradiation, facilitate the generation of ions. The MALDI technology provide a soft ionization for types of molecules or large biomolecules without fragmentation, which can be used for analyzing a wide range of molecules; synthetic small molecules, peptides, proteins, metabolites and polymers; and allowing for accurate molecular mass determination, as well as polydispersity information of polymers. Based on peptide mass fingerprinting (PMF), MS\/MS system equipped high-energy CID (Collision-Induced Dissociation) also enable to produce fragmentation spectra to achieve verification of peptide sequences and protein identification. The device can operate in the linear, reflectron or TOF\/TOF modes, studying both positive and negative ion species. These make it an extremely flexible analytical tool.<\/p>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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\n\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t
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Technical details:<\/p>

Mass range: Linear mode for large molecules up to 500,000Da<\/p>

Resolution: 26,000 FWHM achieved in reflectron mode,\u00a0 1100 FWHM in linear mode<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 3500 FWHM in TOF\/TOF mode<\/p>

Sensitivity: Linear mode:\u00a0 500 fmol BSA (m\/z 66,000)\u00a0 S\/N \u2265 100:1<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Reflectron mode:\u00a0 250 amol peptide (m\/z 1,570.7)\u00a0 S\/N \u2265 100:1<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 MS\/MS mode:\u00a0 250 amol 1570.68Da(Glu-Fib), fragment 1,056 Da at S\/N \u2265 10:1<\/p>

Mass accuracy: Linear < 100 ppm (external calibration);<\/p>

\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Reflectron < 10 ppm (external calibration);\u00a0\u00a0\u00a0\u00a0 2ppm (internal calibration)<\/p>

Mascot search engine: identify protein from primary peptide sequences<\/p>

Applications:<\/p>