{"id":913,"date":"2024-01-23T09:00:18","date_gmt":"2024-01-23T09:00:18","guid":{"rendered":"https:\/\/sites.gtiit.edu.cn\/corefacility\/?page_id=913"},"modified":"2024-03-05T07:31:17","modified_gmt":"2024-03-05T07:31:17","slug":"pt-pcr","status":"publish","type":"page","link":"https:\/\/sites.gtiit.edu.cn\/corefacility\/pt-pcr\/","title":{"rendered":"PT-PCR"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"913\" class=\"elementor elementor-913\" data-elementor-settings=\"[]\">\n\t\t\t\t\t\t<div class=\"elementor-inner\">\n\t\t\t\t\t\t\t<div class=\"elementor-section-wrap\">\n\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9800cfe elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9800cfe\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-16e496c\" data-id=\"16e496c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5f141ba elementor-widget elementor-widget-heading\" data-id=\"5f141ba\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-xl\">PT-PCR<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f33854d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f33854d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-89beb34\" data-id=\"89beb34\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6ece5f7 elementor-widget elementor-widget-image\" data-id=\"6ece5f7\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-image\">\n\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"544\" height=\"444\" src=\"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-content\/uploads\/sites\/74\/2024\/01\/PT-PCR.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c7a2f97 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c7a2f97\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-68676cb\" data-id=\"68676cb\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-43ab29a elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"43ab29a\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-3d23458\" data-id=\"3d23458\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f262931 elementor-widget elementor-widget-text-editor\" data-id=\"f262931\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-text-editor elementor-clearfix\">\n\t\t\t\t<h1><strong>Introduction:<\/strong><\/h1><p>The CFX96 Touch System is a six-channel (five colors and one FRET channel)\u00a0real-time PCR instrument that combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions. Fixed light path technology (six LED lights and six photodiodes) enables optimal detection of dyes in each channel, allowing precise quantification and typing of target genes. You can quickly set up experiments and monitor amplification curves in real time on the integrated LCD touch screen, or you can use the easy-to-use operating software CFX Manager to set up experiments. Samples can be tested using 96-well plates or eight-well plates.<\/p><h1>Application:<\/h1><ul><li>Used for nucleic acid quantification, gene expression level analysis, melting curve-based PCR product specificity analysis, high-resolution melting curve analysis of unknown mutations and SNP sites by saturated fluorescent dye method, etc.<\/li><li>Achieve simultaneous detection of up to 5 target genes<\/li><li>Range of excitation\/emission wavelengths (nm): 450nm &#8211; 730nm<\/li><li>Gradient: 30\u00b0C &#8211; 100\u00b0C (operational range) and 1\u00b0C &#8211; 24\u00b0C (programmable span)<\/li><li>Data analysis modes: standard curve quantification, melting curve, CT or \u0394\u0394CT gene expression analysis, multiple internal reference gene analysis and amplification efficiency calculation, gene expression analysis of multiple data files, allele analysis, endpoint analysis, with alleles , Melting curve analysis function<\/li><\/ul>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e977f5e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e977f5e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0809084\" data-id=\"0809084\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5bab397 elementor-widget elementor-widget-wp-widget-custom_html\" data-id=\"5bab397\" data-element_type=\"widget\" data-widget_type=\"wp-widget-custom_html.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5>Links:<\/h5><div class=\"textwidget custom-html-widget\"><a target=\"_blank\" href=\"http:\/\/sites.gtiit.edu.cn\/corefacility\/wp-content\/uploads\/sites\/74\/2024\/03\/CFX-96-RT-PCR-\u5feb\u901f\u64cd\u4f5c\u6307\u5357.pdf\" rel=\"noopener\">1. CFX-96-RT-PCR-\u5feb\u901f\u64cd\u4f5c\u6307\u5357<\/a>\r\n<br>\r\n<a target=\"_blank\" href=\"http:\/\/sites.gtiit.edu.cn\/corefacility\/wp-content\/uploads\/sites\/74\/2024\/03\/CFX-Maestro\u2122-Software-manual.pdf\" rel=\"noopener\">2. CFX-Maestro\u2122-Software-manual<\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e215f7d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e215f7d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t\t\t<div class=\"elementor-row\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-aa7ad72\" data-id=\"aa7ad72\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-column-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-90a3ebb elementor-widget elementor-widget-button\" data-id=\"90a3ebb\" data-element_type=\"widget\" data-widget_type=\"button.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-button-wrapper\">\n\t\t\t<a href=\"https:\/\/sites.gtiit.edu.cn\/corefacility\/reservation\/\" class=\"elementor-button-link elementor-button elementor-size-md elementor-animation-float\" role=\"button\">\n\t\t\t\t\t\t<span class=\"elementor-button-content-wrapper\">\n\t\t\t\t\t\t<span class=\"elementor-button-icon elementor-align-icon-left\">\n\t\t\t\t<i aria-hidden=\"true\" class=\"far fa-calendar-alt\"><\/i>\t\t\t<\/span>\n\t\t\t\t\t\t<span class=\"elementor-button-text\">GO TO RESERVATION<\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>PT-PCR Introduction: The CFX96 Touch System is a six-channel (five colors and one FRET channel)\u00a0real-time PCR instrument that combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singleplex or multiplex reactions. Fixed light path technology&hellip;&nbsp;<a href=\"https:\/\/sites.gtiit.edu.cn\/corefacility\/pt-pcr\/\" class=\"more-link\">Read More<\/a><\/p>\n","protected":false},"author":375,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":[],"_links":{"self":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/pages\/913"}],"collection":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/users\/375"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/comments?post=913"}],"version-history":[{"count":3,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/pages\/913\/revisions"}],"predecessor-version":[{"id":1453,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/pages\/913\/revisions\/1453"}],"wp:attachment":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/wp-json\/wp\/v2\/media?parent=913"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}