{"id":897,"date":"2024-01-18T07:03:29","date_gmt":"2024-01-18T07:03:29","guid":{"rendered":"https:\/\/sites.gtiit.edu.cn\/corefacility\/?page_id=897"},"modified":"2024-03-05T07:28:53","modified_gmt":"2024-03-05T07:28:53","slug":"facs-fortessa","status":"publish","type":"page","link":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/facs-fortessa\/","title":{"rendered":"LSRFortessa \u7ec6\u80de\u5206\u6790\u4eea"},"content":{"rendered":"
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t

BD LSRFortessa \u7ec6\u80de\u5206\u6790\u4eea<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t\t\t\t\t\t\t\t\t\"\"\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t\t\t\t
\n\t\t\t\t

\u7b80\u4ecb\uff1a<\/strong><\/h1>

BD LSRFortessa \u7ec6\u80de\u5206\u6790\u4eea\u662f\u4e00\u79cd\u6d41\u5f0f\u7ec6\u80de\u672f\u7cfb\u7edf\uff0c\u5728\u514d\u75ab\u5b66\u3001\u7ec6\u80de\u548c\u764c\u75c7\u751f\u7269\u5b66\u9886\u57df\u5177\u6709\u5e7f\u6cdb\u7684\u5e94\u7528\u3002\u5b83\u914d\u5907\u4e865\u79cd\u6fc0\u5149\uff1a\u7d2b\u5916\u7ebf\u3001\u84dd\u8272\u3001\u7ea2\u8272\u3001\u7d2b\u8272\u548c\u9ec4\u7eff\u8272\u3002\u5b83\u6700\u591a\u53ef\u4ee5\u68c0\u6d4b18\u4e2a\u53c2\u6570\u3002\u914d\u5408HTS\u9ad8\u901a\u91cf\u8fdb\u6837\u7cfb\u7edf\uff0c\u53ef\u5b9e\u73b0\u81ea\u52a8\u5feb\u901f\u8fdb\u6837\uff0c\u5728\u751f\u547d\u79d1\u5b66\u7814\u7a76\u4e2d\u53d1\u6325\u91cd\u8981\u4f5c\u7528\u3002<\/p>

\u8981\u786e\u5b9a\u60a8\u7684\u8367\u5149\u67d3\u6599\u662f\u5426\u53ef\u4ee5\u5728 LSRFortessa \u4e0a\u8fdb\u884c\u5206\u6790\uff0c\u8bf7\u53c2\u9605\u4e0b\u8868\uff0c\u6839\u636e\u60a8\u7684\u8367\u5149\u67d3\u6599\u6fc0\u53d1\u548c\u53d1\u5c04\u6765\u786e\u5b9a\u53ef\u4ee5\u4f7f\u7528\u54ea\u4e9b\u8367\u5149\u67d3\u6599\u3002<\/p>

\u8fd9\u4e2a\u300c\u8367\u5149\u5149\u8c31\u67e5\u770b\u5668\u300d<\/a>\u00a0\u5c06\u5e2e\u52a9\u60a8\u786e\u5b9a\u60a8\u60f3\u8981\u4f7f\u7528\u7684\u8367\u5149\u67d3\u6599\u662f\u5426\u6709\u663e\u7740\u7684\u91cd\u53e0\u3002<\/p>

\u7279\u6027\uff1a<\/h1>

Laser<\/strong><\/p><\/td>

Detector<\/strong><\/p><\/td>

Name<\/strong><\/p><\/td>

Filter<\/strong><\/p><\/td>

Mirror<\/strong><\/p><\/td>

Some common fluorochromes<\/strong><\/p><\/td><\/tr>

UV (355nm)<\/strong><\/p><\/td>

A<\/strong><\/p><\/td>

Indo-1(Blue)<\/strong><\/p><\/td>

530\/30<\/strong><\/p><\/td>

505LP<\/strong><\/p><\/td>

DAPI, Hoechst, Indo-1(unbound), BFP,AF350<\/strong><\/p><\/td><\/tr>

B<\/strong><\/p><\/td>

Indo-1(Violet)<\/strong><\/p><\/td>

450\/50<\/strong><\/p><\/td>

NIL<\/strong><\/p><\/td>

Indo-1(free),BUV496, AF350, DAPI, Hoechst, BFP, Indo-1 (free)<\/strong><\/p><\/td><\/tr>

Violet (405nm)<\/strong><\/p><\/td>

A<\/strong><\/p><\/td>

BV786<\/strong><\/p><\/td>

780\/60<\/strong><\/p><\/td>

750LP<\/strong><\/p><\/td>

BV785,BV786, Qdot800(26%),SuperBright780<\/strong><\/p><\/td><\/tr>

B<\/strong><\/p><\/td>

BV711<\/strong><\/p><\/td>

710\/50<\/strong><\/p><\/td>

685LP<\/strong><\/p><\/td>

BV711, eFluro 700NC, Qdot705(28%),SuperBright702<\/strong><\/p><\/td><\/tr>

C<\/strong><\/p><\/td>

BV650<\/strong><\/p><\/td>

660\/20<\/strong><\/p><\/td>

630LP<\/strong><\/p><\/td>

BV650, , eFluro 650NC, Qdot655(38%), SuperBright780<\/strong><\/p><\/td><\/tr>

D<\/strong><\/p><\/td>

BV605<\/strong><\/p><\/td>

610\/20<\/strong><\/p><\/td>

595LP<\/strong><\/p><\/td>

BV605, BV570, eFluro 605NC, Qdot605(38%), Qdot 585(29%), SuperBright 600<\/strong><\/p><\/td><\/tr>

E<\/strong><\/p><\/td>

BV510<\/strong><\/p><\/td>

525\/50<\/strong><\/p><\/td>

505LP<\/strong><\/p><\/td>

BV510, BV480, AmCyan, Aqua, CFP, e506, Qdot 525, V500, PacOrange, Alexa Fluor 430<\/strong><\/p><\/td><\/tr>

F<\/strong><\/p><\/td>

BV421<\/strong><\/p><\/td>

450\/40<\/strong><\/p><\/td>

NIL<\/strong><\/p><\/td>

AF405, BFP, BV421, DAPI, Dye Cycle Violet, e450, Hoechst, V450<\/strong><\/p><\/td><\/tr>

Blue (488nm)<\/strong><\/p><\/td>

A<\/strong><\/p><\/td>

PerCP-Cy5-5<\/strong><\/p><\/td>

710\/50<\/strong><\/p><\/td>

685LP<\/strong><\/p><\/td>

BB700, PerCP, PerCP-Cy5.5, 7-AAD<\/strong><\/p><\/td><\/tr>

B<\/strong><\/p><\/td>

FITC<\/strong><\/p><\/td>

530\/30<\/strong><\/p><\/td>

505LP<\/strong><\/p><\/td>

BB515, AF488, FITC, GFP, Zombie Green<\/strong><\/p><\/td><\/tr>

C<\/strong><\/p><\/td>

SSC<\/strong><\/p><\/td>

488\/10<\/strong><\/p><\/td>

NIL<\/strong><\/p><\/td>

SSC<\/strong><\/p><\/td><\/tr>

YellowGreen (561 nm)<\/strong><\/p><\/td>

A<\/strong><\/p><\/td>

PE-Cy7<\/strong><\/p><\/td>

780\/60<\/strong><\/p><\/td>

750LP<\/strong><\/p><\/td>

PE-Cy7, PE-AF750<\/strong><\/p><\/td><\/tr>

B<\/strong><\/p><\/td>

PE-Cy5-5<\/strong><\/p><\/td>

710\/50<\/strong><\/p><\/td>

685LP<\/strong><\/p><\/td>

PE-Cy5.5, PE-AF700<\/strong><\/p><\/td><\/tr>

C<\/strong><\/p><\/td>

PE-Cy5<\/strong><\/p><\/td>

670\/30<\/strong><\/p><\/td>

635LP<\/strong><\/p><\/td>

7AAD, Katushka, mPlum, PE-Cy5<\/strong><\/p><\/td><\/tr>

D<\/strong><\/p><\/td>

PE-Texas Red<\/strong><\/p><\/td>

610\/20<\/strong><\/p><\/td>

600LP<\/strong><\/p><\/td>

PE-Texas Red, mCherry, PI, AF568, PE-CF594<\/strong><\/p><\/td><\/tr>

E<\/strong><\/p><\/td>

PE<\/strong><\/p><\/td>

586\/15<\/strong><\/p><\/td>

NIL<\/strong><\/p><\/td>

PE, tdTomato, dsRed, CY3,RFP<\/strong><\/p><\/td><\/tr>

Red (640nm)<\/strong><\/p><\/td>

A<\/strong><\/p><\/td>

APC-Cy7<\/strong><\/p><\/td>

780\/60<\/strong><\/p><\/td>

750LP<\/strong><\/p><\/td>

APC-Cy7, APC-H7, AF750<\/strong><\/p><\/td><\/tr>

B<\/strong><\/p><\/td>

APC <\/strong><\/p><\/td>

730\/45<\/strong><\/p><\/td>

690LP<\/strong><\/p><\/td>

AF700, APC-R700, DyeCycleRuby<\/strong><\/p><\/td><\/tr>

C<\/strong><\/p><\/td>

AlexaFluor 700<\/strong><\/p><\/td>

670\/14<\/strong><\/p><\/td>

NIL<\/strong><\/p><\/td>

AF647, APC,Cy5, SytoxRed<\/strong><\/p><\/td><\/tr><\/tbody><\/table>\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t
Link:<\/h5>
1. BD-LSRFortessa-Users-Guide<\/a>\r\n
\r\n2. BD-FACSFortessa\u4e2d\u6587\u7b80\u6613\u64cd\u4f5c.pdf<\/a>\r\n
\r\n3. BD-HTS-Users-Guide<\/a><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t
\n\t\t\t\t\t\t\t
\n\t\t\t\t\t\t
\n\t\t\t\t
\n\t\t\t\t\t
\n\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t\t\t\n\t\t\t\t<\/i>\t\t\t<\/span>\n\t\t\t\t\t\t\u524d\u5f80\u9884\u7ea6<\/span>\n\t\t<\/span>\n\t\t\t\t\t<\/a>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"

BD LSRFortessa Cell Analyzer Introduction: BD LSRFortessa cell analyzer is a flow cytometry system with a wide range of applications in immunology, cell and cancer biology.\u00a0It is equipped with 5 lasers: UV, blue, red, violet, and yellow green. It can… Read More<\/a><\/p>","protected":false},"author":375,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":[],"_links":{"self":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/pages\/897"}],"collection":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/users\/375"}],"replies":[{"embeddable":true,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/comments?post=897"}],"version-history":[{"count":3,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/pages\/897\/revisions"}],"predecessor-version":[{"id":1448,"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/pages\/897\/revisions\/1448"}],"wp:attachment":[{"href":"https:\/\/sites.gtiit.edu.cn\/corefacility\/zh\/wp-json\/wp\/v2\/media?parent=897"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}