Shimadzu HPLC LC-20 series

High-performance liquid chromatography (HPLC) is a widely used and highly powerful chromatographic technique, particularly suited for separating low-volatility or non-volatile organic compounds. Our HPLC systems are equipped with both UV–Vis and refractive index (RI) detectors, enabling the analysis of a broad range of chromophoric and non-chromophoric compounds. Typical applications include the analysis of aromatics, alcohols, small organic molecules, simple sugars, polysaccharides, large biomolecules (such as proteins, peptides, and antibodies), as well as certain polymers.

We have also established a chiral HPLC system for the separation of enantiomers and the determination of enantiomeric excess (ee) in chiral compounds. A variety of polysaccharide-based chiral columns are available, offering specific selectivity for amines, acids, amides, esters, alcohols, sulfoxides, and arylpropionic acid derivatives.

Technical Specifications

  • Maximum pressure: 40 MPa
  • Flow rate: Up to 10 mL/min
  • Gradient capability: High-pressure gradient mixing.
  • Autosampler Injection volume: 0.1 to 100 uL.
  • Oven temperature control: 10°C to 100°C in 1°C steps.
  • UV-Vis detector with dual-wavelength mode, Wavelength range: 190 to 700nm
  • RID: refractive index of 1 to 1.75 RIU.