Bruker Autoflex Max LRF Maldi-TOF
The Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) is a powerful mass spectrometer with a rapid, accurate, and high-resolution technique. The MALDI process involves sample molecules crystallising with a matrix compound, which upon laser irradiation, facilitate the generation of ions. The MALDI technology provide a soft ionization for types of molecules or large biomolecules without fragmentation, which can be used for analyzing a wide range of molecules; synthetic small molecules, peptides, proteins, metabolites and polymers; and allowing for accurate molecular mass determination, as well as polydispersity information of polymers. Based on peptide mass fingerprinting (PMF), MS/MS system equipped high-energy CID (Collision-Induced Dissociation) also enable to produce fragmentation spectra to achieve verification of peptide sequences and protein identification. The device can operate in the linear, reflectron or TOF/TOF modes, studying both positive and negative ion species. These make it an extremely flexible analytical tool.
Technical details:
Mass range: Linear mode for large molecules up to 500,000Da
Resolution: 26,000 FWHM achieved in reflectron mode, 1100 FWHM in linear mode
3500 FWHM in TOF/TOF mode
Sensitivity: Linear mode: 500 fmol BSA (m/z 66,000) S/N ≥ 100:1
Reflectron mode: 250 amol peptide (m/z 1,570.7) S/N ≥ 100:1
MS/MS mode: 250 amol 1570.68Da(Glu-Fib), fragment 1,056 Da at S/N ≥ 10:1
Mass accuracy: Linear < 100 ppm (external calibration);
Reflectron < 10 ppm (external calibration); 2ppm (internal calibration)
Mascot search engine: identify protein from primary peptide sequences
Applications:
- Organic molecules, peptides, proteins, nucleic acids, lipids and sugars MW determination
- Proteomics, Peptide mass fingerprinting
- Protein top-down sequencing (without enzymatic digestion)
- Polymer characterization
- Isotopic distribution of transition metals and their complexes
Sample requirements: nonvolatile compounds