Laser

Detector

Name

Filter

Mirror

Some common fluorochromes

UV (355nm)

A

Indo-1(Blue)

530/30

505LP

DAPI, Hoechst, Indo-1(unbound), BFP,AF350

B

Indo-1(Violet)

450/50

NIL

Indo-1(free),BUV496, AF350, DAPI, Hoechst, BFP, Indo-1 (free)

Violet (405nm)

A

BV786

780/60

750LP

BV785,BV786, Qdot800(26%),SuperBright780

B

BV711

710/50

685LP

BV711, eFluro 700NC, Qdot705(28%),SuperBright702

C

BV650

660/20

630LP

BV650, , eFluro 650NC, Qdot655(38%), SuperBright780

D

BV605

610/20

595LP

BV605, BV570, eFluro 605NC, Qdot605(38%), Qdot 585(29%), SuperBright 600

E

BV510

525/50

505LP

BV510, BV480, AmCyan, Aqua, CFP, e506, Qdot 525, V500, PacOrange, Alexa Fluor 430

F

BV421

450/40

NIL

AF405, BFP, BV421, DAPI, Dye Cycle Violet, e450, Hoechst, V450

Blue (488nm)

A

PerCP-Cy5-5

710/50

685LP

BB700, PerCP, PerCP-Cy5.5, 7-AAD

B

FITC

530/30

505LP

BB515, AF488, FITC, GFP, Zombie Green

C

SSC

488/10

NIL

SSC

YellowGreen (561 nm)

A

PE-Cy7

780/60

750LP

PE-Cy7, PE-AF750

B

PE-Cy5-5

710/50

685LP

PE-Cy5.5, PE-AF700

C

PE-Cy5

670/30

635LP

7AAD, Katushka, mPlum, PE-Cy5

D

PE-Texas Red

610/20

600LP

PE-Texas Red, mCherry, PI, AF568, PE-CF594

E

PE

586/15

NIL

PE, tdTomato, dsRed, CY3,RFP

Red (640nm)

A

APC-Cy7

780/60

750LP

APC-Cy7, APC-H7, AF750

B

APC

730/45

690LP

AF700, APC-R700, DyeCycleRuby

C

AlexaFluor 700

670/14

NIL

AF647, APC,Cy5, SytoxRed