Model:
Thermo Scientific UltiMate 3000
Location:
R402
Main parameters:
- Maximum pressure: 62 Mpa (620 bar, 9000 psi)
- Solvent channels: 1 or 4
- Detector data acquisition rate: 100 Hz maximum
Typical applications:
HPLC takes liquid as the mobile phase and adopts a high-pressure infusion system to pump mobile phases such as single solvent with different polarity or mixed solvents and buffers with different ratios into the chromatographic column equipped with stationary phases, and then after the components in the column are separated, it enters into the detector for detection to realize the analysis of the specimen. It is mainly used for the analysis of high boiling point non-volatile, thermally unstable, and large molecular weight organic compounds, which are divided into qualitative and quantitative. HPLC can solve the following problems: the analysis and determination of non-volatile compounds; the analysis and determination of polar compounds; the analysis and determination of thermally unstable compounds; and the analysis and determination of large molecular weight compounds (including proteins, peptides, and polymers, etc.).
Sample requirements:
Powder samples need to be supplied with more than 20 mg and liquid samples need to be supplied with more than 0.5 mL, with good solubility in conventional solvents.
