enzyme-catalyzed reactions kinetics, inhibition and cooperativity, immobilized enzymes, introduction to metabolic control analysis. flux balance in metabolic networks. systems-level aspects of metabolism and genetic regulation. restriction enzymes and their use in genetic engineering. expression vectors and cloning. dna libraries. dna sequencing, amplification, mutagenesis. applications of genetic engineering to medicine. growth stochiometry. microorganisms and their cultivation. population growth kinetics. biological reactors. heat and mass transfer in bio-reactors. genetic stability in population and in reactors.

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